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Electrochemiluminescence detection of glucose-oxidase as a model for flow-injection immunoassays

Robert Wilson, Jens Kremeskotter, David J.Schiffrin and James S.Wilkinson*

Department of Chemistry, University of Liverpool, P.O.Box 147, Liverpool L69 3BX, UK.
* Optoelectronics Research Centre, University of Southampton, UK

Abstract

Glucose oxidase was covalently attached to an aminosilanized indium tin oxide coated glass wafer with the hydroxysuccinimide ester of pyridyldithiopropionic acid. The wafer was used as the working electrode in the reaction chamber of a flow injection analyser. When a solution of glucose and luminol was passed over the electrode, glucose was oxidized enzymatically and luminol was oxidized electro-chemically. The products of these reactions generated light in proportion to the amount of glucose in the sample. When glucose was assayed in the range 0-10 mM the detection limit was 0.419 mM with a correlation coefficient of 0.9962.


Biosensors & Bioelectronics (1996) Vol.11(8) pp.805-810

doi: 10.1016/0956-5663(96)85932-X

Southampton ePrint id: 78164

 

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Copyright University of Southampton 2006