The University of Southampton
University of Southampton Institutional Repository

Nanoparticle-enhanced chemiluminescence in micro-flow injection analysis

Nanoparticle-enhanced chemiluminescence in micro-flow injection analysis
Nanoparticle-enhanced chemiluminescence in micro-flow injection analysis
Chemiluminescence (CL) detection for biomedical analysis has the principal advantage that no optical source is required so that instrumentation is simple and background radiation is minimised, resulting in high sensitivity. CL has been exploited in a wide range of chemical and biochemical measurements such as enzyme-linked immunoassays (ELISA), DNA sequencing, and for the analysis of biomedical, food and environmental samples [1]. CL is ideally suited to microfluidic flow-injection analysis (µFIA), due to the precise temporal and space control of sample/reagent aliquots [2]. However, while CL is a sensitive technique, the ultrasmall volumes employed in µFIA lead to low emitted power, so that signal enhancement methods are required to achieve suitable detection limits. Gold and silver nanoparticles (GNPs and SNPs) may provide enhancement of optical signals due to collective oscillation of conduction electrons excited by the electromagnetic field or due to catalysis [3,4]. In this study, CL of luminol was investigated in a microflow chip with a serpentine channel of width 600 µm, depth 75 µm and length 150 mm formed in polydimethylsiloxane (PDMS) by moulding over a 3D printed master.
Mosayyebi, Ali
ab9cf6da-58c4-4441-993b-7d03d5d3549a
Karabchevsky, Alina
26a2c158-ef26-43be-8f10-b37e04baa017
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Mosayyebi, Ali
ab9cf6da-58c4-4441-993b-7d03d5d3549a
Karabchevsky, Alina
26a2c158-ef26-43be-8f10-b37e04baa017
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca

Mosayyebi, Ali, Karabchevsky, Alina and Wilkinson, J.S. (2013) Nanoparticle-enhanced chemiluminescence in micro-flow injection analysis. 6th Mediterranean Conference on Nano-Photonics (MediNano-6), Lyon, France. 30 - 31 Oct 2013.

Record type: Conference or Workshop Item (Paper)

Abstract

Chemiluminescence (CL) detection for biomedical analysis has the principal advantage that no optical source is required so that instrumentation is simple and background radiation is minimised, resulting in high sensitivity. CL has been exploited in a wide range of chemical and biochemical measurements such as enzyme-linked immunoassays (ELISA), DNA sequencing, and for the analysis of biomedical, food and environmental samples [1]. CL is ideally suited to microfluidic flow-injection analysis (µFIA), due to the precise temporal and space control of sample/reagent aliquots [2]. However, while CL is a sensitive technique, the ultrasmall volumes employed in µFIA lead to low emitted power, so that signal enhancement methods are required to achieve suitable detection limits. Gold and silver nanoparticles (GNPs and SNPs) may provide enhancement of optical signals due to collective oscillation of conduction electrons excited by the electromagnetic field or due to catalysis [3,4]. In this study, CL of luminol was investigated in a microflow chip with a serpentine channel of width 600 µm, depth 75 µm and length 150 mm formed in polydimethylsiloxane (PDMS) by moulding over a 3D printed master.

Text
6117.pdf - Other
Download (240kB)

More information

Published date: 2013
Venue - Dates: 6th Mediterranean Conference on Nano-Photonics (MediNano-6), Lyon, France, 2013-10-30 - 2013-10-31
Organisations: Optoelectronics Research Centre

Identifiers

Local EPrints ID: 361187
URI: http://eprints.soton.ac.uk/id/eprint/361187
PURE UUID: e8f106c1-fdf7-40db-8971-efadabee1e7d
ORCID for Ali Mosayyebi: ORCID iD orcid.org/0000-0003-0901-6546
ORCID for J.S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 15 Jan 2014 13:55
Last modified: 28 Mar 2024 02:51

Export record

Contributors

Author: Ali Mosayyebi ORCID iD
Author: Alina Karabchevsky
Author: J.S. Wilkinson ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×