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Nanoparticle-enhanced chemiluminescence in micro-flow injection analysis

Ali Mosayyebi, Alina Karabchevsky and James S.Wilkinson


Chemiluminescence (CL) detection for biomedical analysis has the principal advantage that no optical source is required so that instrumentation is simple and background radiation is minimised, resulting in high sensitivity. CL has been exploited in a wide range of chemical and biochemical measurements such as enzyme-linked immunoassays (ELISA), DNA sequencing, and for the analysis of biomedical, food and environmental samples [1]. CL is ideally suited to microfluidic flow-injection analysis (μFIA), due to the precise temporal and space control of sample/reagent aliquots [2]. However, while CL is a sensitive technique, the ultrasmall volumes employed in μFIA lead to low emitted power, so that signal enhancement methods are required to achieve suitable detection limits. Gold and silver nanoparticles (GNPs and SNPs) may provide enhancement of optical signals due to collective oscillation of conduction electrons excited by the electromagnetic field or due to catalysis [3,4]. In this study, CL of luminol was investigated in a microflow chip with a serpentine channel of width 600 μm, depth 75 μm and length 150 mm formed in polydimethylsiloxane (PDMS) by moulding over a 3D printed master.

6th Mediterranean Conference on Nano-Photonics (MediNano-6) Lyon France 30-31 Oct (2013)

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