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Multi-modal particle manipulator to enhance bead-based bioassays

Multi-modal particle manipulator to enhance bead-based bioassays
Multi-modal particle manipulator to enhance bead-based bioassays
By sequentially pushing micro-beads towards and away from a sensing surface, we show that ultrasonic radiation forces can be used to enhance the interaction between a functionalized glass surface and polystyrene micro-beads, and distinguish those that bind to the surface, ultimately by using an integrated optical waveguide implanted in the reflector to facilitate optical detection. The movement towards and immobilization of streptavidin coated beads onto a biotin functionalized waveguide surface is achieved by using a quarter-wavelength mode pushing beads onto the surface, while the removal of non-specifically bound beads uses a second quarter-wavelength mode which exhibits a kinetic energy maxima at the boundary between the carrier layer and fluid, drawing beads towards this surface. This has been achieved using a multi-modal acoustic device which exhibits both these quarter-wavelength resonances. Both 1-D acoustic modelling and finite element analysis has been used to design this device and investigate the spatial uniformity of the field. We demonstrate experimentally that 90% of specifically bound beads remain attached after applying ultrasound, with 80% of non-specifically bound control beads being successfully removed acoustically. This approach overcomes problems associated with lengthy sedimentation processes used for bead-based bioassays and surface (electrostatic) forces, which delay or prevent immobilisation. We explain the potential of this technique in the development of DNA and protein assays in terms of detection speed and multiplexing.
acoustic radiation forces, bio-sensor, frequency switching, micro-beads, optical waveguide
Glynne-Jones, P.
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Boltryk, R.J.
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Hill, M.
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Zhang, F.
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Dong, L.
4a30a247-d676-42ef-aaf4-061579d6d64e
Wilkinson, J.S.
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Brown, T.
a64aae36-bb30-42df-88a2-11be394e8c89
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Harris, N.R.
237cfdbd-86e4-4025-869c-c85136f14dfd
Glynne-Jones, P.
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Boltryk, R.J.
0452b21c-a758-4d4a-925b-1511d9296d62
Hill, M.
0cda65c8-a70f-476f-b126-d2c4460a253e
Zhang, F.
396ca776-f0e6-4750-974a-6ba3f9c78a41
Dong, L.
4a30a247-d676-42ef-aaf4-061579d6d64e
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Brown, T.
a64aae36-bb30-42df-88a2-11be394e8c89
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Harris, N.R.
237cfdbd-86e4-4025-869c-c85136f14dfd

Glynne-Jones, P., Boltryk, R.J., Hill, M., Zhang, F., Dong, L., Wilkinson, J.S., Brown, T., Melvin, T. and Harris, N.R. (2009) Multi-modal particle manipulator to enhance bead-based bioassays. International Congress on Ultrasonics, Santiago, Chile. 11 - 17 Jan 2009.

Record type: Conference or Workshop Item (Paper)

Abstract

By sequentially pushing micro-beads towards and away from a sensing surface, we show that ultrasonic radiation forces can be used to enhance the interaction between a functionalized glass surface and polystyrene micro-beads, and distinguish those that bind to the surface, ultimately by using an integrated optical waveguide implanted in the reflector to facilitate optical detection. The movement towards and immobilization of streptavidin coated beads onto a biotin functionalized waveguide surface is achieved by using a quarter-wavelength mode pushing beads onto the surface, while the removal of non-specifically bound beads uses a second quarter-wavelength mode which exhibits a kinetic energy maxima at the boundary between the carrier layer and fluid, drawing beads towards this surface. This has been achieved using a multi-modal acoustic device which exhibits both these quarter-wavelength resonances. Both 1-D acoustic modelling and finite element analysis has been used to design this device and investigate the spatial uniformity of the field. We demonstrate experimentally that 90% of specifically bound beads remain attached after applying ultrasound, with 80% of non-specifically bound control beads being successfully removed acoustically. This approach overcomes problems associated with lengthy sedimentation processes used for bead-based bioassays and surface (electrostatic) forces, which delay or prevent immobilisation. We explain the potential of this technique in the development of DNA and protein assays in terms of detection speed and multiplexing.

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Microsoft_Word_-_ICU_Manuscript_Boltryk.pdf - Accepted Manuscript
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More information

Published date: January 2009
Venue - Dates: International Congress on Ultrasonics, Santiago, Chile, 2009-01-11 - 2009-01-17
Keywords: acoustic radiation forces, bio-sensor, frequency switching, micro-beads, optical waveguide

Identifiers

Local EPrints ID: 70918
URI: http://eprints.soton.ac.uk/id/eprint/70918
PURE UUID: eee2d381-246e-4733-944b-eca3c2b5ce6a
ORCID for P. Glynne-Jones: ORCID iD orcid.org/0000-0001-5684-3953
ORCID for M. Hill: ORCID iD orcid.org/0000-0001-6448-9448
ORCID for J.S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697
ORCID for N.R. Harris: ORCID iD orcid.org/0000-0003-4122-2219

Catalogue record

Date deposited: 11 Mar 2010
Last modified: 14 Mar 2024 02:43

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Contributors

Author: P. Glynne-Jones ORCID iD
Author: R.J. Boltryk
Author: M. Hill ORCID iD
Author: F. Zhang
Author: L. Dong
Author: J.S. Wilkinson ORCID iD
Author: T. Brown
Author: T. Melvin
Author: N.R. Harris ORCID iD

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