Fabrication and testing of a micro-fabricated PCR Device with an integrated fluorescence detector
Fabrication and testing of a micro-fabricated PCR Device with an integrated fluorescence detector
In this paper, we report the complete microfabrication of a polymerase chain reaction (PCR) device with both integrated temperature cycling and fluorescence detection elements. Also experimental data of temperature cycling and fluorescence detection system which includes an integrated photodiode covered by a thin film of cadmium sulphide as an optical filter are presented. Experiments indicate that temperature cycling is much faster than conventional PCR thermocycler also the composition of the photodiode and optical filter produces enough attenuation at excitation wavelength for fluorescence detection.
Bahrami, M.
9120e4d5-cbab-40fe-ac61-290ec1cb262a
Evans, A.G.R.
082f720d-3830-46d7-ba87-b058af733bc3
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Husband, B.
88ce6797-521a-4cd2-88ca-0d8182cb9876
Sessions, N.P.
ee737092-56b4-403e-a2f9-764e07e42625
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
September 2005
Bahrami, M.
9120e4d5-cbab-40fe-ac61-290ec1cb262a
Evans, A.G.R.
082f720d-3830-46d7-ba87-b058af733bc3
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Husband, B.
88ce6797-521a-4cd2-88ca-0d8182cb9876
Sessions, N.P.
ee737092-56b4-403e-a2f9-764e07e42625
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Bahrami, M., Evans, A.G.R., Wilkinson, J.S., Husband, B., Sessions, N.P. and Melvin, T.
(2005)
Fabrication and testing of a micro-fabricated PCR Device with an integrated fluorescence detector.
16th MicroMechanics Europe Workshop (MME 2005), Gothenburg, Sweden.
03 - 05 Sep 2005.
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Conference or Workshop Item
(Paper)
Abstract
In this paper, we report the complete microfabrication of a polymerase chain reaction (PCR) device with both integrated temperature cycling and fluorescence detection elements. Also experimental data of temperature cycling and fluorescence detection system which includes an integrated photodiode covered by a thin film of cadmium sulphide as an optical filter are presented. Experiments indicate that temperature cycling is much faster than conventional PCR thermocycler also the composition of the photodiode and optical filter produces enough attenuation at excitation wavelength for fluorescence detection.
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Published date: September 2005
Venue - Dates:
16th MicroMechanics Europe Workshop (MME 2005), Gothenburg, Sweden, 2005-09-03 - 2005-09-05
Identifiers
Local EPrints ID: 57736
URI: http://eprints.soton.ac.uk/id/eprint/57736
PURE UUID: f295c190-1f35-4721-9cf1-07bf9ec8039e
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Date deposited: 11 Aug 2008
Last modified: 12 Dec 2021 02:32
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Contributors
Author:
M. Bahrami
Author:
A.G.R. Evans
Author:
B. Husband
Author:
N.P. Sessions
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