Photo-patterning of DNA oligonucleotides on silicon surfaces with micron-scale dimensions
Photo-patterning of DNA oligonucleotides on silicon surfaces with micron-scale dimensions
A method is presented for the covalent attachment of oligonucleotides to silicon (100) surfaces patterned with micron-scale features. UV light exposure of hydrogen-terminated silicon (100) coated with alkenes functionalized with N-hydroxysuccinimide ester groups results in Si-C bonded monolayers. The N-hydroxysuccinimide ester surfaces act as a template for the subsequent covalent attachment of DNA oligonucleotides. In order to create patterns of surface attached DNA oligonucleotides with high density, the surface attachment chemistry has been investigated and optimised. Micron scale patterning of surfaces was achieved by exposure with UV laser light via a mask. DNA oligonucleotide patterns, with feature sizes of several microns, were reliably produced over large areas. The patterned surfaces were characterised with scanning electron microscopy, epifluorescence microscopy and ellipsometry. Hybridisation with fluorescent label- and gold nanoparticle-conjugates of the complementary oligonucleotide is achieved. The methods offer reliable approaches for the creation of micron-scale motifs of DNA on surfaces.
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Yin, Huabing B.
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Brown, Tom
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Greef, Robert
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Mailis, Sakellaris
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Eason, Rob W.
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Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Melvin, Tracy
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Yin, Huabing B.
2e1000f5-7da9-4164-a3c1-3a81e3359f9b
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Greef, Robert
91063544-ed89-4738-b5f4-28a984bf5866
Mailis, Sakellaris
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Eason, Rob W.
e38684c3-d18c-41b9-a4aa-def67283b020
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Yin, Huabing B., Brown, Tom, Greef, Robert, Mailis, Sakellaris, Eason, Rob W., Wilkinson, James S. and Melvin, Tracy
(2004)
Photo-patterning of DNA oligonucleotides on silicon surfaces with micron-scale dimensions.
Biophotonics New Frontier: From Genome to Proteome, Strasbourg, France.
27 Apr 2004.
.
(doi:10.1117/12.545736).
Record type:
Conference or Workshop Item
(Paper)
Abstract
A method is presented for the covalent attachment of oligonucleotides to silicon (100) surfaces patterned with micron-scale features. UV light exposure of hydrogen-terminated silicon (100) coated with alkenes functionalized with N-hydroxysuccinimide ester groups results in Si-C bonded monolayers. The N-hydroxysuccinimide ester surfaces act as a template for the subsequent covalent attachment of DNA oligonucleotides. In order to create patterns of surface attached DNA oligonucleotides with high density, the surface attachment chemistry has been investigated and optimised. Micron scale patterning of surfaces was achieved by exposure with UV laser light via a mask. DNA oligonucleotide patterns, with feature sizes of several microns, were reliably produced over large areas. The patterned surfaces were characterised with scanning electron microscopy, epifluorescence microscopy and ellipsometry. Hybridisation with fluorescent label- and gold nanoparticle-conjugates of the complementary oligonucleotide is achieved. The methods offer reliable approaches for the creation of micron-scale motifs of DNA on surfaces.
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e-pub ahead of print date: September 2004
Additional Information:
(SPIE 5461)
Venue - Dates:
Biophotonics New Frontier: From Genome to Proteome, Strasbourg, France, 2004-04-27 - 2004-04-27
Identifiers
Local EPrints ID: 13917
URI: http://eprints.soton.ac.uk/id/eprint/13917
PURE UUID: 6cdbb58f-9e97-45c5-9d6b-722931c9a0c9
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Date deposited: 07 Mar 2005
Last modified: 16 Mar 2024 02:37
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Contributors
Author:
Huabing B. Yin
Author:
Robert Greef
Author:
Sakellaris Mailis
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