Oligonucleotide-conjugated thiazole orange probes as "light-up" probes for messenger ribonucleic acid molecules in living cells
Oligonucleotide-conjugated thiazole orange probes as "light-up" probes for messenger ribonucleic acid molecules in living cells
"Light-up" probes, icosa-α-thymidylate-thiazole orange conjugates, for the in situ time-resolved detection of messenger ribonucleic acid (mRNA) in living cells are evaluated. Upon annealing with polyA in aqueous solutions, the icosa-alpha-thymidylate-thiazole orange conjugates were shown to be up to 15 times more fluorescent. Microinjection of these probes into adherent fibroblasts resulted in high yields of hybridization and fluorescent signals. Incubation of cells in the presence of these probes resulted in facile internalization of the probe and similar painting of the messenger RNA in the nuclear and cytosolic regions.
532-541
Privat, Eric
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Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Asseline, Ulysse
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Vigny, Paul
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October 2001
Privat, Eric
cd59645e-cb14-494f-bfa0-eb5fa5474d73
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Asseline, Ulysse
543d0dd8-2d4f-44fb-a9e3-7a4d6795d704
Vigny, Paul
d06c1221-9686-4f05-872b-454d5f6cdee5
Privat, Eric, Melvin, Tracy, Asseline, Ulysse and Vigny, Paul
(2001)
Oligonucleotide-conjugated thiazole orange probes as "light-up" probes for messenger ribonucleic acid molecules in living cells.
Photochemistry and Photobiology, 74 (4), .
Abstract
"Light-up" probes, icosa-α-thymidylate-thiazole orange conjugates, for the in situ time-resolved detection of messenger ribonucleic acid (mRNA) in living cells are evaluated. Upon annealing with polyA in aqueous solutions, the icosa-alpha-thymidylate-thiazole orange conjugates were shown to be up to 15 times more fluorescent. Microinjection of these probes into adherent fibroblasts resulted in high yields of hybridization and fluorescent signals. Incubation of cells in the presence of these probes resulted in facile internalization of the probe and similar painting of the messenger RNA in the nuclear and cytosolic regions.
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Published date: October 2001
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Local EPrints ID: 41236
URI: http://eprints.soton.ac.uk/id/eprint/41236
ISSN: 0031-8655
PURE UUID: a40cf06b-401e-4557-8844-646a1c5d675b
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Date deposited: 04 Aug 2006
Last modified: 08 Jan 2022 03:55
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Author:
Eric Privat
Author:
Ulysse Asseline
Author:
Paul Vigny
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